Effects of bovine spermatozoa preparation on embryonic development in vitro

نویسندگان

  • Marko Samardzija
  • Martina Karadjole
  • Iva Getz
  • Zdenko Makek
  • Marijan Cergolj
  • Tomislav Dobranic
چکیده

The aim of our research was to examine the ability of density gradient preparation BoviPure and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared before and after sperm processing using BoviPure and swim up methods. We also evaluated and compared cleavage rates, embryo yield and quality between the methods. There were significant differences (P < 0.05) between the sperm characteristics before and after BoviPure, but not after swim up method. However, there were significant differences for sperm results among those two mentioned methods. A total of 641 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA. The percentage of cleavage (Day 2) and the percentage of hatched embryos (Day 9) were similar for both methods. However, embryo production rate (Day 7) was significantly higher using BoviPure method (P < 0.05). Also, total cell number and embryo differential staining (inner cell mass and trophectoderm cells) of Day 7 morulas and blastocysts showed that BoviPure treated sperm displayed higher quality embryos compared to swim up method (P < 0.05). Our results indicate that BoviPure method has an enhanced capacity in sperm selection for in vitro embryo production when compared with swim up method. So, we concluded that BoviPure could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Various Activators on Bovine Embryonic Development Following Intracytoplasmic Sperm Injection

The objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (ICSI) in terms of cleavage and embryo development. Bovine ICSI oocytes were treated with a variation of treatments of combined or single activators as follows; 30 V for 15 x 2 μsec of electrical pulse +10 μg/mL cycloheximide (CHX),7% ethanol...

متن کامل

Spermatozoa Molecules in Relation to Bulls Fertility

Bull fertility may be defined as the process by which spermatozoa fertilize and activate the ovum and then support embryonic development. Bull fertility is a complex trait having relatively low heritability and plays a vital role for efficient production and reproduction of bovine. Various mechanisms involved in regulating bull fertility associated phenotype and reliable biomarkers are poorly d...

متن کامل

Effect of Ethanol on Maturation and Development of Immatur Mouse Oocytes

Purpose: To study the effect of ethanol on resumption of meiosis, maturation and development of immature mouse oocytes in vitro. Materials and Methods: Immature oocytes were dissected from ovary of NMRI strain mouse (6-8 weeks old) and divided into control and experimental groups. The absolute ethanol was imposed to oocytes at doses of 0 (Control), 0.05, 0.1, 0.2 and 0.4% (v/v) in maturation c...

متن کامل

Impacts of in vitro thermal stress on ovine epididymal spermatozoa and the protective effect of β-mercaptoethanol as an antioxidant

Most aspects of reproductive function including spermatogenesis, oocyte growth and maturation, early embryonic development, fetal and placental growth, and lactation can be affected by thermal stress. Furthermore, it has been shown that oxidative stress involves in the pathology of thermal stress. Therefore, the aim of this study was to investigate the impacts of thermal stress on the ovine mat...

متن کامل

Effects of sera and steroid hormones on development of bovine oocytes matured and fertilized in vitro and co-cultured with bovine oviduct epithelial cells.

The present experiment was designed to identify possible effects of sera and steroid hormones added to a co-culture with bovine oviduct epithelial cells on embryonic development in vitro. Bovine oocytes were matured in vitro for 24 h and then fertilized in vitro using swim-up and heparin-treated, frozen-thawed spermatozoa. At 18 and 20 h after insemination, oocytes were cultured for 3 or 7 d in...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Reproductive Biology and Endocrinology

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2006